We started our day with “Microbial Ecology of the Norman Landfill” lecture by Dr. Suflita and “Animal-Microbe-Symbioses by Anne Dunn. Both lecture discussed new things and it was pretty cool. In lab, we jot down the color of plates we inoculated last Friday. My plate has numerous growth and glad to see the result. Next, we start taking phenol and vanillan data and also checked the growth of test-tubes. At last, we get to know Q-FAME technique that is related to fatty acids. We also did gel electrophoresis and took cool snaps of DNA. Yay!!!!
First day of week 2, the lecture by Dr. Dunn was amazing! I enjoyed every slide and am looking forward to hearing Blake’s speech about quorum sensing; can’t wait. Part II (lab) was covered by running gel and learning about FAME. 4 more days to go… we can do it ppl J
I enjoyed the lecture from Dr. Anne Dunn( http://faculty-staff.ou.edu/D/Anne.K.Dunn-1/) about Vibrio fischeri and bioluminescence and symbiosis demonstrated in the Hawaiian bobtail squid (Euprymna scolopes). Josph Suflita, Director of the OU Institute for Energy and the Environment, gave a very interesting presentation over the Norman Landfill research site from a metabolomic perspec tive. We went to the lab after lunch for some PCR, PAGE, and a demonstration of the Q-FAME Rapid Microbial ID Method of the landfill aquifer samples.
It is hard to believe it is already August and only a week of this course has gone by. For some reason I was really awake today and actually understanding what was going on. One of the lectures today talked about the landfill, and pretty much what we are doing, but put it more into perspective. The second lecture spoke about the symbiotic relationship between a squid in haiwaii and a luminescent bacteria. Very interesting stuff! Labwork today made time fly by! I’m still not an expert pipetter, but I did learn I’ve been pipetting wrong for a whole week. Whoops! Phenol and vanillan degradation is happening faster than I expected, but it could level off and slow down eventually. I hope that doesn’t happen cause it is the one experiment I am really interested in. I didn’t think it was possible for a mathematician to become a scientist, but I extracted DNA today. There is a first time for everything and this week has been full of them, but not very many people can say they have extracted DNA from an organism. Well guess what, I have! Not by myself, but I have! Ready to see what tomorrow will bring.