I have never been in the lab this late. I am having a lot of fun, but I know tomorrow this late night is going to hit me. I did have a lot of fun today. Dr Cichewicz spoke to us about Race, genetics, and health. His lecture talked a lot about drugs and pharmaceuticals. Dr Lawson, the poo doctor, spoke with us microbial taxonomy and how molecular biology has affected it. After lunch I extracted environmental DNA from LF-2B leachate. Judith and I then used the PCR technique to amplify the archaeal DNA. Blake ran the gels and we repeated the Phenol and Vanillan degradation as a class. We then used the TOPO method to incorporate our DNA into a plasmid so that the bacteria could reproduce the DNA for us. I am currently waiting for Blake to return with the cells so that I can plate them and go home to sleep…hurry Blake!
We had two speakers today: Dr. Cichewicz and Dr. Lawson (who I got to introduce in front of the class). It was a very new experience. Dr. Cichewicz talked about some interesting scope in a field of microbiology as a career. Also in the lab, we did some interesting experiment by running PCR, gel making, and TOPO cloning reaction. Overall it was very long and yet very educational day!
The longest day so far. 9 A.M. to 11:20 P.M. It was a long and intense day of 2 lectures before lunch and PCR, Agarose Gel Electrophoresis, Topo Cloning ,and Transformation of Ecoli strain Mach 1 all using the Power Biofilm kit from Invitrogen. The kit worked great! Pushing through the day was worth it because we learned so many new subjects and molecular techniques while completing our experiments and this will give us enough time to collect and analyze data so that we will be able create our poster and present our findings on Fri, Aug 5th and is my b-day. If you want to give me a gift, donate some money to this program or to EPSCOR). Yes….I said DATA! We started to collect absorbance readings that, when analyzed, will tell us if we have come closer to isolating Phenol and Vanillin degrading microorganisms